Targeted photoswitchable imaging of intracellular glutathione by a photochromic glycosheet sensor

• Xianzhi Chai,
• Hai-Hao Han,
• Yi Zang,
• Jia Li,
• Xiao-Peng He,
• Junji Zhang and
• He Tian

Beilstein J. Org. Chem. 2019, 15, 2380–2389, doi:10.3762/bjoc.15.230

• therapy. Keywords: intracellular GSH; molecular switches; photochromic glycosheet; photoswitchable imaging; 2D MnO2 nanosheets; Introduction Cells are the basic structure and functional unit of biological organisms. Human diseases and aging are closely related to the states of cells. Thorough

• design and further broaden the availability of photoswitchable biosensing, herein we report a glycosheet hybrid sensor (Glyco-DTE@MnO2) fabricated by 2D MnO2 nanosheets and dithienylethene fluorescence reporter (Glyco-DTE) to achieve cell-targeted photoswitchable imaging of intracellular GSH. As shown in

• Scheme 1, Glyco-DTE@MnO2 glycosheets were formed by assembling Glyco-DTE onto the surface of 2D MnO2 nanosheets, which quench the fluorescence from Glyco-DTE reporter. Recent studies discovered that 2D MnO2 nanosheets tend to undergo a facile reduction with GSH, MnO2 + 2GSH + 2H+ → Mn2+ + GSSG + 2H2O [21